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Journal: Current biology : CB

Article Title: Microtubule growth rates are sensitive to global and local changes in microtubule plus-end density

doi: 10.1016/j.cub.2020.05.056

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins Leupeptin EMD Millipore Cat#EI8 Pepstatin EMD Millipore Cat#EI10; CAS: 26305-03-3 Chymostatin EMD Millipore Cat#EI6 Cycloheximide Sigma-Aldrich Cat#C7698; CAS: 66-81-9 Cytochalasin D Sigma-Aldrich Cat#C6762; CAS: 14930-96-2 PMSG Prospec-Tany Technogene Cat#hor-272; Lot#718PGSMP hCG Prospec-Tany Technogene Cat#hor-250; Lot#119PHCG13 SYLGARD 184 Silicone elastomer Dow Corning Material Number 99109937 3-(trimethoxysilyl)propyl methacrylate Sigma-Aldrich Cat#440159 CAS: 2530-85-0 Poly(ethylene glycol) methacrylate Sigma-Aldrich Cat#409537 CAS: 25736-86-1 Poly(ethylene glycol) diacrylate Sigma-Aldrich Cat#455008 CAS: 26570-48-9 Lithium phenyl-2,4,6-trimethylbenzoylphosphinate Sigma-Aldrich Cat#900889 Pico-Surf Sphere Fluidics SKU: C021 EB1-GFP Gatlin Lab N/A mCherry-TMBD Gatlin Lab [ 13 ] N/A Experimental Models: Organisms/Strains Xenopus laevis adult female NASCO https://www.enasco.com/c/Education-Supplies/Xenopus-Frogs Software and Algorithms Metamorph 7.7 software Molecular Devices https://www.moleculardevices.com/products/cellular-imaging-systems/acquisition-and-analysis-software/metamorph-microscopy ImageJ National Institutes of Health https://imagej.net/Fiji/Downloads MATLAB MathWorks https://www.mathworks.com/products/matlab.html Excel Microsoft https://products.office.com/en-us/excel u-track Danuser Lab https://github.com/DanuserLab/u-track Local density analysis Gatlin Lab http://dx.doi.org/10.17632/fj8rrmgwny.1 IgorPro WaveMetrics https://www.wavemetrics.com/products/igorpro Open in a separate window KEY RESOURCES TABLE

Techniques: Recombinant, Software